Selenoprotein W antibody

Catalog No. ABIN401399

The Rabbit Polyclonal anti-Selenoprotein W antibody is suitable to detect Selenoprotein W in samples from Rat and Mouse. It has been validated for WB and EIA.

Quick Overview for Selenoprotein W antibody (ABIN401399)

Target: Selenoprotein W (SEPW1) (Selenoprotein W, 1 (SEPW1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Selenoprotein W antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Selenoprotein W Antibody

Specificity: This antibody is specific for Selenoprotein W.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Purification: Affinity chromatography

Immunogen: Recombinant protein corresponding to full-length mouse SelW protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/100,000. Western Blot: 1/1,000 - 1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.96 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Selenoprotein W

Target: Selenoprotein W (SEPW1) (Selenoprotein W, 1 (SEPW1))

Alternative Name: Selenoprotein W (SELW)

Background: Selenoprotein W was first purified and characterized from rat skeletal muscle. The function of selenoprotein W is not entirely clear, but the presence of the bound glutathione moiety indicates that SelW is thought to function in oxidation-reduction catalysis and may play a role in selenium deficiency disorders such as white muscle disease in sheep and Keshan disease in humans. Recently, overexpression of SelW was shown to be glutathione dependent and was shown to markedly reduce the sensitivity of cell lines to H2O2 cytotoxicity.Synonyms: SEPW, SEPW1

Gene ID: 20364

NCBI Accession: NP_033182

UniProt: P63300

Pathways: Cell RedoxHomeostasis