FAP antibody (Internal Region)

Catalog No. ABIN401429

The Rabbit Polyclonal anti-FAP antibody is suitable to detect FAP in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for FAP antibody (Internal Region) (ABIN401429)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAP antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FAP Antibody

Binding Specificity: Internal Region

Specificity: This antibody is known to react with Mouse FAP protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Human.
Species reactivity (tested):Mouse.

Purification: Immunoffinity Chromatography.

Immunogen: Synthetic peptide corresponding to an internal region of mouse FAP protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/100000. Western Blot: 1/500-1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.65 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: Seprase / FAP

Background: Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.Synonyms: 170 kDa melanoma membrane-bound gelatinase, Fibroblast activation protein alpha, Integral membrane serine protease

Gene ID: 14089

NCBI Accession: NP_032012

UniProt: P97321

Pathways: Tube Formation