PKC beta antibody (Internal Region)

Catalog No. ABIN401431

The Rabbit Polyclonal anti-PKC beta antibody is suitable to detect PKC beta in samples from Human, Rat, Mouse, Monkey, Rabbit and Cow. It has been validated for WB and EIA.

Quick Overview for PKC beta antibody (Internal Region) (ABIN401431)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivity: Human, Rat, Mouse, Monkey, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC beta antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PKC beta Antibody

Binding Specificity: Internal Region

Specificity: This antibody is specific for PKC beta protein.

Cross-Reactivity (Details): Species reactivity (expected):Rabbit, Bovine, Rat, Mouse.
Species reactivity (tested):Human, Monkey.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to an internal region of human Protein kinase C beta

Isotype: IgG

Application Details

Application Notes: ELISA: 1/75,000 - 1/80,000. Western Blot: 1/1,000 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Az

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PRKCB

Background: Protein kinase C (PKC) beta is implicated as a potential important factor in cancer cell proliferation and angiogenesis. PKC is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.Synonyms: PKC beta, PKC-B, PKC-beta, PKCB, PRKCB1, Protein kinase C beta type

Gene ID: 5579

NCBI Accession: NP_002729

UniProt: P05771

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling