V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB) antibody

Antigen: V-Myb Myeloblastosis Viral Oncogene Homolog (Avian) (MYB)

Synonyms: efg, Cmyb, c-myb, c-myb_CDS, MYB

Clonality: Polyclonal

Host: Rabbit

Reactivity: Chimpanzee

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN401456

Additional Information

Characteristics: Synonyms: c-Myb, Proto-oncogene c-Myb, Transcriptional activator Myb

Alternative name: MYB

Swiss-Prot: P06876

Immunogen: Peptide corresponding to an internal portion of the c-Myb protein

Cross-Reactivity: Chimpanzee, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus), Bonobo

Isotype: IgG  (Matching secondary antibodies)

Description: The transcription factor c-Myb plays an important role in the development of allhematopoietic lineages during definitive hematopoiesis. Its critical role in hematopoiesishas been first demonstrated in experiments with targeted disruption of the c-Myb genewhere deletion of both alleles of the c-Myb gene was embryonally lethal due to severeanemia. c-Myb is a short-lived sequence specific DNA-binding protein that regulatestranscription of several important genes involved directly in cellular processes such asproliferation, differentiation, and apoptosis. Posttranslational modifications such asphosphorylation, acetylation, ubiquitination, and conjugation of SUMO proteins are crucialfor modulation of the transactivation activity of c-Myb.

Specificity: This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressedc-Myb protein in transfected Cos7 cells. Species: Rat, Mouse, Porcine, Human, Horse, Platypus, Opossum, Orangutan, Spidermonkey, Bonobo, Gorilla, Chimpanzee. Others not tested.

Application Details

Application Notes: ELISA: 1/10,000 – 1/25,000. Western Blot: 1/250 – 1/1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Purification: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. Storage Conditions for Trial Size: This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volumedilute 1: 10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mixthoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use thisintermediate dilution when calculating final dilutions as recommended below. Shelf life: 3 month from despatch.

Restrictions: For Research Use only