Sorting Nexin 26 (SNX26) (C-Term) antibody

Details for Product No. ABIN401527
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Synonyms SNX26, tcgap, NOMA-GAP, TCGAP, AW324378, Snx26
(18), (16), (1)
Human, Mouse (Murine)
(37), (1)
(34), (3)
(2), (2), (2), (2), (2), (2)
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(29), (18), (12), (9), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN401527
368.50 $
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Immunogen Peptide from the C Terminus of the protein sequence according to NP_443180.2. Genename: SNX26
Specificity This antibody detects SNX26 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):MouseSpecies reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name Sorting Nexin-26 (SNX26)
Background SNX26 may be involved in several stages of intracellular trafficking. It could play an important role in the regulation of glucose transport by insulin. It may act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.Synonyms: ARHGAP33, Rho GTPase-activating protein 33, TCGAP
Gene ID 115703
NCBI Accession NP_443180
UniProt O14559
Application Notes Peptide ELISA: antibody detection limit dilution 1: 8000. Western blot: Preliminary experiments gave an approx 75 kDa band in Human Bone Marrow,Kidney, and Pancreas lysates after 1 μg/mL antibody staining. ABIN401527 may detect theisoform A1A5D2 (UniProtKB) with a molecular weight of 81.5 kDa which is similar in aminoacid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently wecannot find a precise explanation in the literature for the band we observe given thecalculated size of 125 kDa according to NP_443180.2. The 75 kDa band was successfullyblocked by incubation with the immunizing peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?Immunohistochemistry on paraffin sections: 2.5 μg/mL. In Human Brain shows cytoplasmstaining of Purkinje cells in Cerebellum.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
anti-Sorting Nexin 26 (SNX26) (C-Term) antibody anti-Sorting Nexin 26 (SNX26) (C-Term) antibody
Background publications Teasdale, Loci, Houghton et al.: "A large family of endosome-localized proteins related to sorting nexin 1." in: The Biochemical journal, Vol. 358, Issue Pt 1, pp. 7-16, 2001 (PubMed).

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