GATA3 antibody (N-Term)

Catalog No. ABIN401530

This Goat Polyclonal antibody specifically detects GATA3 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig and Dog and has been mentioned in 3+ publications.

Quick Overview for GATA3 antibody (N-Term) (ABIN401530)

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This GATA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GATA3 Antibody

Binding Specificity: N-Term

Sequence: EVTADQPRWV SHH-C

Specificity: This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3

Application Details

Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-GATA3 antibody (ABIN401530)

Lee, Bae, Seo, Cho, Bae, Oh, Park: "Mycoleptodonoides aitchisonii suppresses asthma via Th2 and Th1 cell regulation in an ovalbumin‑induced asthma mouse model." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 11-20, (2018) (PubMed).

Seo, Bang, Kim, Cho, Park: "Erythronium japonicum attenuates histopathological lung abnormalities in a mouse model of ovalbumin-induced asthma." in: International journal of molecular medicine, Vol. 37, Issue 5, pp. 1221-8, (2016) (PubMed).

Bang, Seo, Seo, Jo, Jung, Yu, Park, Park: "Bacillus subtilis KCTC 11782BP-Produced Alginate Oligosaccharide Effectively Suppresses Asthma via T-Helper Cell Type 2-Related Cytokines." in: PLoS ONE, Vol. 10, Issue 2, pp. e0117524, (2015) (PubMed).

Target Details for GATA3

Target: GATA3 (GATA Binding Protein 3 (GATA3))

Alternative Name: GATA3

Background: The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3

Gene ID: 2625

NCBI Accession: NP_001002295

UniProt: P23771

Pathways: Hormone Transport, Regulation of Hormone Metabolic Process, Tube Formation