GATA Binding Protein 3 (GATA3) (N-Term) antibody

Details for Product No. ABIN401530
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Antigen
Synonyms GATA-3, XGATA3, hdr, xGATA-3, GATA3, gata3, HDR, HDRS, xgata-3, Gata-3, jal, cb550, gta3
Epitope
N-Term
(21), (12), (12), (12), (11), (10), (8), (6), (5), (3), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(133), (90), (52), (52), (49), (48), (40), (38), (8), (1), (1), (1)
Host
Goat
(106), (28), (10), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1)
Application
ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(99), (57), (40), (30), (25), (22), (12), (3), (2), (1)
Pubmed 2 references available
Quantity 0.1 mg
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Catalog No. ABIN401530
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Immunogen Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3
Sequence EVTADQPRWV SHH-C
Specificity This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name GATA3
Background The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
Alternate names: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
Gene ID 2625
NCBI Accession NP_001002295
UniProt P23771
Research Area Lineage Markers, Transcription Factors, Adaptive Immunity
Application Notes Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3µg/ml. Approx 50kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.9kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3µg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH~7.3 0.02% Sodium Azide 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
Expiry Date 12 months
Supplier Images
anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody
anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody (2) anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody (Image 2)
Background publications Marine, Winoto: "The human enhancer-binding protein Gata3 binds to several T-cell receptor regulatory elements." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 7284-8, 1991 (PubMed).

Van Opstal, Hepp, Suzuki et al.: "Role of monkey nucleus reticularis tegmenti pontis in the stabilization of Listing's plane." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 22, pp. 7284-96, 1996 (PubMed).

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