ZFPM1 antibody (Middle Region)

Catalog No. ABIN404786

There is 1 publication for this product available. The Rabbit Polyclonal anti-ZFPM1 antibody is suitable to detect ZFPM1 in samples from Human, Rat and Cow. It has been validated for WB.

Quick Overview for ZFPM1 antibody (Middle Region) (ABIN404786)

Target: ZFPM1 (Zinc Finger Protein, Multitype 1 (ZFPM1))

Reactivity: Human, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZFPM1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZFPM1 Antibody

Binding Specificity: Middle Region

Purpose: ZFPM1 Antibody - middle region

Sequence: PPPPPPGHAP APESPRPGSG SGSGPGLAPA RSPGPAADGP IDLSKKPRRP

Predicted Reactivity: Cow: 91%, Human: 100%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against ZFPM1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZFPM1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP32193-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZFPM1 antibody (ABIN404786)

Sugiyama, Tanaka, Kitajima, Zheng, Yen, Murotani, Yamatodani, Nakano: "Differential context-dependent effects of friend of GATA-1 (FOG-1) on mast-cell development and differentiation." in: Blood, Vol. 111, Issue 4, pp. 1924-32, (2008) (PubMed).

Target Details for ZFPM1

Target: ZFPM1 (Zinc Finger Protein, Multitype 1 (ZFPM1))

Alternative Name: ZFPM1

Background:

Background Information: ZFPM1 is a transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. ZFPM1 is an essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. ZFPM1 may be involved in regulation of some genes in gonads. ZFPM1 may also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2.

Gene Name: Zinc finger protein, multitype 1

Alternative Symbols: FOG, FOG1, ZNF408, ZNF89A, ZC2HC11A

Protein Name: Zinc finger protein ZFPM1

Molecular Weight: 105kDa

Gene ID: 161882

NCBI Accession: NP_722520

UniProt: Q8IX07