Myeloid Cell Nuclear Differentiation Antigen (MNDA) antibody

Antigen: Myeloid Cell Nuclear Differentiation Antigen (MNDA)

Synonyms: PYHIN3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN405140

Additional Information

Alternative name: MNDA

Gene ID: MNDA

UniProt: P41218

Immunogen: The immunogen for anti-MNDA antibody: synthetic peptide directed towards the C terminal of human MNDA

Sequence: KCEKGDKLRLFCLQLRTVDRKLKLVCGSHSFIKVIKAKKN KEGPMNVN

Cross-Reactivity: Human

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-MNDA antibody concentration is 1 mg/ml.

Description: The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage.MNDA may act as a transcriptional activator/repressor in the myeloid lineage. It plays a role in the granulocyte/monocyte cell-specific response to interferon. MNDA stimulates the DNA binding of the transcriptional repressor protein YY1.The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Characteristics: Antigen Size: 407 amino acids

Molecular Weight: 46kDa

Application Details

Application Notes: WB Suggested Anti-MNDA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Transfected 293T.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Research Area: Stem Cells, Hematopoietic Progenitors, Innate Immunity, Cytokines

Restrictions: For Research Use only

Images

WB Suggested Anti-MNDA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Transfected 293T

Publications

Product: Briggs, Shults, Flye et al.: "Dysregulated human myeloid nuclear differentiation antigen expression in myelodysplastic syndromes: evidence for a role in apoptosis." in: Cancer research, Vol. 66, Issue 9, pp. 4645-51, 2006 (PubMed).