ATP6V0A2 antibody (N-Term)

Catalog No. ABIN405464

The Rabbit Polyclonal anti-ATP6V0A2 antibody has been validated for WB and IHC. It is suitable to detect ATP6V0A2 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Saccharomyces cerevisiae and Zebrafish (Danio rerio).

Quick Overview for ATP6V0A2 antibody (N-Term) (ABIN405464)

Target: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATP6V0A2 Antibody

Binding Specificity: N-Term

Sequence: INRADIPLPE GEASPPAPPL KQVLEMQEQL QKLEVELREV TKNKEKLRKN

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 83%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 92%, Rat: 100%, Yeast: 79%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against ATP6V0A2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP6V0A2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 856 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ATP6V0A2

Target: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Alternative Name: ATP6V0A2

Background: The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain, ATP6V0A2 is a component of the V(0) domain.The multisubunit vacuolar-type proton pump (H(+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H(+)-ATPases are comprised of a peripheral V(1) domain and an integral membrane V(0) domain, ATP6V0A2 is a component of the V(0) domain (Smith et al., 2003 [PubMed 14580332]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: ATP6N1D, ATP6a2, J6B7, Stv1, TJ6, TJ6M, TJ6s, Vph1, a2, A2, RTF, WSS, ARCL, STV1, TJ6S, VPH1, ARCL2A, ATP6A2
Protein Interaction Partner: UBC, RPL10L, PTRH2, ATP6V1D, RPS15, RPS2, SLC25A3, AKT1, ELAVL1, CYTH2,
Protein Size: 856

Molecular Weight: 98 kDa

Gene ID: 23545

NCBI Accession: NM_012463, NP_036595

UniProt: Q9Y487

Pathways: Transition Metal Ion Homeostasis, Proton Transport