ATP6V0A2 antibody (AA 72-121)

Catalog No. ABIN6741595

The Rabbit Polyclonal anti-ATP6V0A2 antibody (ABIN6741595) specifically detects ATP6V0A2 in WB, IHC and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ATP6V0A2 antibody (AA 72-121) (ABIN6741595)

Target: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V0A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATP6V0A2 Antibody

Binding Specificity: AA 72-121

Specificity: Human ATP6V0A2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat (100%) Dog, Horse (92%) Pig (91%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa72-121 of human ATP6V0A2 (Q9Y487, NP_036595). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat (100%), Monkey, Galago, Elephant, Dog, Bat, Horse (92%), Pig (91%), Bovine, Platypus (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for ATP6V0A2

Target: ATP6V0A2 (ATPase, H+ Transporting, Lysosomal V0 Subunit A2 (ATP6V0A2))

Alternative Name: ATP6V0A2

Background: Name/Gene ID: ATP6V0A2
Subfamily: ATPase - V type
Family: Transporter

Synonyms: ATP6V0A2, A2V-ATPase, ARCL, ARCL2A, ATP6A2, ATP6N1D, RTF, STV1, V-ATPase 116 kDa, TJ6S, WSS, J6B7, TJ6, TJ6M, V-ATPase 116 kDa isoform a2, VPH1

Gene ID: 23545

NCBI Accession: NP_036595

UniProt: Q9Y487

Pathways: Transition Metal Ion Homeostasis, Proton Transport