Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4) (N-Term) antibody

Details for Product No. ABIN405545
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms BC056494, mKIAA1399, MARCH-IV, RNF174
Epitope
N-Term
(23), (15), (9), (5), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(62), (23), (17), (4), (4), (3), (3), (2), (2), (2), (1), (1)
Host
Rabbit
(48), (15), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(35), (32), (11), (10), (8), (3), (1)
Pubmed 1 reference available
Quantity 50 μg
Options
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Immunogen Synthetic peptide directed towards the N terminal of human MARCH4
Sequence GLLKCRCRML FNDLKVFLLR RPPQAPLPMH GDPQPPGLAA NNTLPALGAG
Predicted Reactivity Human :100 %,   Rat :80 %,   Mouse :80 %,  
Characteristics This is a rabbit polyclonal antibody against MARCH4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MARCH4 (MARCH4 Antibody Abstract)
Background MARCH4 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Molecular Weight 45 kDa
Gene ID 57574
NCBI Accession NM_020814, NP_065865
UniProt Q9P2E8
Research Area Proteolysis / Ubiquitin
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 410 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4) (N-Term) antibody WB Suggested Anti-MARCH4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Bartee, Mansouri, Hovey Nerenberg et al.: "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins." in: Journal of virology, Vol. 78, Issue 3, pp. 1109-20, 2004 (PubMed).

Validation Images
Catalog No. ABIN405545
289.00 $
Plus shipping costs $45.00
Quantity
Price
50 μg
289.00 $

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)
Did you look for something else?