Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4) (N-Term) antibody

Details for Product No. ABIN405545
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Antigen
Synonyms BC056494, mKIAA1399, MARCH-IV, RNF174
Epitope
N-Term
(8), (7), (3), (2), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Human
(41), (25), (19), (19), (18), (17), (17), (2), (1), (1), (1)
Host
Rabbit
(36), (4), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(18), (17), (15), (10), (7), (6), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN405545
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Immunogen Synthetic peptide directed towards the N terminal of human MARCH4
Sequence GLLKCRCRML FNDLKVFLLR RPPQAPLPMH GDPQPPGLAA NNTLPALGAG
Predicted Reactivity Human :100 %,   Rat :80 %,   Mouse :80 %,  
Characteristics This is a rabbit polyclonal antibody against MARCH4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MARCH4
Background MARCH4 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Molecular Weight 45 kDa
Gene ID 57574
NCBI Accession NM_020814, NP_065865
UniProt Q9P2E8
Research Area Proteolysis / Ubiquitin
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 410 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4) (N-Term) antibody WB Suggested Anti-MARCH4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Hela cell lysate
Background publications Bartee, Mansouri, Hovey Nerenberg et al.: "Downregulation of major histocompatibility complex class I by human ubiquitin ligases related to viral immune evasion proteins." in: Journal of virology, Vol. 78, Issue 3, pp. 1109-20, 2004 (PubMed).

Validation Images
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