GOT2 antibody (N-Term)

Catalog No. ABIN405573

Cited in 2+ publications. The Rabbit Polyclonal anti-GOT2 antibody (ABIN405573) specifically detects GOT2 in WB. The antibody is reactive with Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse, Rabbit, Dog, Guinea Pig and Saccharomyces cerevisiae samples.

Quick Overview for GOT2 antibody (N-Term) (ABIN405573)

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse, Rabbit, Dog, Guinea Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GOT2 Antibody

Binding Specificity: N-Term

Purpose: GOT2 Antibody - N-terminal region

Sequence: VEMGPPDPIL GVTEAFKRDT NSKKMNLGVG AYRDDNGKPY VLPSVRKAEA

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GOT2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GOT2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP43517-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GOT2 antibody (ABIN405573)

Povlsen, Løfgren, Dalgas, Birkler, Johannsen, Støttrup, Bøtker: "Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation." in: PLoS ONE, Vol. 8, Issue 5, pp. e64093, (2013) (PubMed).

Tsai, Hong, Liou, Liao: "Association study of GOT2 genetic polymorphisms and schizophrenia." in: Psychiatric genetics, Vol. 17, Issue 5, pp. 314, (2007) (PubMed).

Target Details for GOT2

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Alternative Name: GOT2

Background:

Background Information: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2)

Alternative Symbols: KAT4, DEE82, KATIV, KYAT4, mitAAT

Protein Name: Aspartate aminotransferase, mitochondrial

Molecular Weight: 45kDa

Gene ID: 2806

NCBI Accession: NP_002071

UniProt: P00505

Pathways: Monocarboxylic Acid Catabolic Process