Anoctamin 10 antibody (C-Term)

Catalog No. ABIN405905

The Rabbit Polyclonal anti-Anoctamin 10 antibody has been validated for WB. It is suitable to detect Anoctamin 10 in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio). There are 4+ publications available.

Quick Overview for Anoctamin 10 antibody (C-Term) (ABIN405905)

Target: Anoctamin 10 (ANO10)

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anoctamin 10 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Anoctamin 10 Antibody

Binding Specificity: C-Term

Sequence: LKADIDATLY EQVILEKEMG TYLGTFDDYL ELFLQFGYVS LFSCVYPLAA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against TMEM16K. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TMEM16K

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 660 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Anoctamin 10 antibody (ABIN405905)

Wanitchakool, Ousingsawat, Sirianant, Cabrita, Faria, Schreiber, Kunzelmann: "Cellular defects by deletion of ANO10 are due to deregulated local calcium signaling." in: Cellular signalling, Vol. 30, pp. 41-49, (2016) (PubMed).

Schreiber, Kunzelmann: "Expression of anoctamins in retinal pigment epithelium (RPE)." in: Pflügers Archiv : European journal of physiology, Vol. 468, Issue 11-12, pp. 1921-1929, (2016) (PubMed).

Hammer, Wanitchakool, Sirianant, Papiol, Monnheimer, Faria, Ousingsawat, Schramek, Schmitt, Margos, Michel, Kraiczy, Pawlita, Schreiber, Schulz, Fingerle, Tumani, Ehrenreich, Kunzelmann: "A Coding Variant of ANO10, Affecting Volume Regulation of Macrophages, Is Associated with Borrelia Seropositivity." in: Molecular medicine (Cambridge, Mass.), Vol. 21, pp. 26-37, (2015) (PubMed).

Tian, Schreiber, Kunzelmann: "Anoctamins are a family of Ca2+-activated Cl- channels." in: Journal of cell science, Vol. 125, Issue Pt 21, pp. 4991-8, (2013) (PubMed).

Target Details for Anoctamin 10

Target: Anoctamin 10 (ANO10)

Alternative Name: TMEM16K

Background: TMEM16K is a multi-pass membrane protein. It belongs to the anoctamin family. TMEM16K may act as a calcium-activated chloride channel.
Alias Symbols: FLJ10375, MGC47890, SCAR10, TMEM16K
Protein Interaction Partner: UBC,
Protein Size: 660

Molecular Weight: 76 kDa

Gene ID: 55129

NCBI Accession: NM_018075, NP_060545

UniProt: Q9NW15