PDHB antibody (N-Term)

Catalog No. ABIN405971

The Rabbit Polyclonal anti-PDHB antibody has been validated for WB and IHC. It is suitable to detect PDHB in samples from Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for PDHB antibody (N-Term) (ABIN405971)

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDHB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-PDHB Antibody

Binding Specificity: N-Term

Sequence: GLWKKYGDKR IIDTPISEMG FAGIAVGAAM AGLRPICEFM TFNFSMQAID

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PDHB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PDHB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 359 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: PDHB

Background: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Alias Symbols: DKFZp564K0164, PHE1B, PDHBD
Protein Interaction Partner: HUWE1, SUMO2, SUMO3, STAU1, MDM2, RNF2, RRAGB, YWHAE, SNRPD1, PCYT2, EIF5, ADRB2, ITGB1, GNB2, FN1, FCER1A, MDC1, ECT2, PDHX, PDHA1, OGDH, RPSA, FBXO6, CAND1, CUL3, UBC, TSC22D1, RAP1B, PFDN1, GRB7, RCC1, CDKN1A, ANXA7, DYRK2, SRRM2, TRIM63, PDHA2, PDHB,
Protein Size: 359

Molecular Weight: 39 kDa

Gene ID: 5162

NCBI Accession: NM_000925, NP_000916

UniProt: P11177

Pathways: Warburg Effect