GNAO1 antibody (Middle Region)

Catalog No. ABIN406520

There is 1 publication for this product available. The Rabbit Polyclonal anti-GNAO1 antibody is suitable to detect GNAO1 in samples from Mouse, Human, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Sheep and Saccharomyces cerevisiae. It has been validated for WB and IHC.

Quick Overview for GNAO1 antibody (Middle Region) (ABIN406520)

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Reactivity: Mouse, Human, Rat, Cow, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Sheep, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GNAO1 Antibody

Binding Specificity: Middle Region

Sequence: CDVVSRMEDT EPFSAELLSA MMRLWGDSGI QECFNRSREY QLNDSAKYYL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against GNAO1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GNAO1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 354 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GNAO1 antibody (ABIN406520)

Zupancic, Blejec, Herman, Veber, Verbovsek, Korsic, Knezevic, Rozman, Turnsek, Gruden, Motaln: "Identification of plasma biomarker candidates in glioblastoma using an antibody-array-based proteomic approach." in: Radiology and oncology, Vol. 48, Issue 3, pp. 257-66, (2014) (PubMed).

Target Details for GNAO1

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Alternative Name: GNAO1

Background: Activated Goalpha interacted directly with PLZF, and enhanced its function as a transcriptional and cell growth suppressor. Goalpha might play a role in mediating extracellular signal-regulated kinase activation by G protein-coupled receptors in the brain.
Alias Symbols: DKFZp686O0962, G-ALPHA-o, GNAO
Protein Interaction Partner: UBC, ADRA2A, BAG3, Htt, RGS9, GABBR1, Haus4, RGS1, RIC8A, DCTN2, KLHL3, SCN8A, PDE6G, CRHR1, GPSM2, HRH4, NGB, S1PR5, RGS19, GRM6, FFAR2, TSHR, RGS5, RGS10, RGS7, RGS4, RGS16, RGS14, PTPRU, CCR5, NUCB1, PDE6H, CACNA1B, OPRM1, OPRD1, GNAO1, GNAI1, ADRA2C,
Protein Size: 354

Molecular Weight: 40 kDa

Gene ID: 2775

NCBI Accession: NM_138736, NP_620073

UniProt: B3KP89

Pathways: G-protein mediated Events