GNAO1 antibody (Subunit alpha)

Catalog No. ABIN462341

The Rabbit Polyclonal anti-GNAO1 antibody is suitable to detect GNAO1 in samples from Mouse and Drosophila melanogaster. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for GNAO1 antibody (Subunit alpha) (ABIN462341)

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Reactivity: Mouse, Drosophila melanogaster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GNAO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GNAO1 Antibody

Binding Specificity: AA 345-354, Subunit alpha

Specificity: Recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kD forming a heterotrimeric protein complex with beta and gamma subunits. Two isoforms of the alpha subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2. Has been used successfully for the detection of G(o) in Drosophila by western blotting.

Purification: Ammonium sulfate precipitation

Immunogen: Synthetic peptide from mouse GNAO1 / GNAO.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:500 - 1:1000), IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: This antibody detects band at 39 kD when used under reducing conditions against rat brain.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for GNAO1

Target: GNAO1 (Guanine Nucleotide Binding Protein (G Protein), alpha Activating Activity Polypeptide O (GNAO1))

Alternative Name: GNAO1

Background: Name/Gene ID: GNAO1

Synonyms: GNAO1, GNAO, G-ALPHA-o, G0 Protein Alpha

Gene ID: 2775

UniProt: P09471

Pathways: G-protein mediated Events