GTF2H2 antibody (N-Term)

Catalog No. ABIN406779

This anti-GTF2H2 antibody is a Rabbit Polyclonal antibody detecting GTF2H2 in WB. Suitable for Human, Mouse, Rat, Horse, Zebrafish (Danio rerio) and Dog.

Quick Overview for GTF2H2 antibody (N-Term) (ABIN406779)

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Reactivity: Human, Mouse, Rat, Horse, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2H2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GTF2H2 Antibody

Binding Specificity: N-Term

Sequence: DILFKAKRKR VFEHHGQVRL GMMRHLYVVV DGSRTMEDQD LKPNRLTCTL

Cross-Reactivity: Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against GTF2H2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GTF2H2

Application Details

Application Notes: WB Suggested Anti-GTF2H2 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 395 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GTF2H2

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Alternative Name: GTF2H2 / BTF2P44

Background: GTF2H2 gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. GTF2H2 is the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs, however, no sequence data is currently available for the centromeric copy of the gene.This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This gene is within the telomeric copy of the duplication. Deletion of this gene sometimes accompanies deletion of the neighboring SMN1 gene in spinal muscular atrophy (SMA) patients but it is unclear if deletion of this gene contributes to the SMA phenotype. This gene encodes the 44 kDa subunit of RNA polymerase II transcription initiation factor IIH which is involved in basal transcription and nucleotide excision repair. Transcript variants for this gene have been described, but their full length nature has not been determined. A second copy of this gene within the centromeric copy of the duplication has been described in the literature. It is reported to be different by either two or four base pairs, however, no sequence data is currently available for the centromeric copy of the gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-15 BM083743.1 1-15 16-1121 AF078847.1 1-1106 1122-1537 BG283896.1 128-543 1538-1951 AC044797.5 132110-132523 c
Alias Symbols: BTF2, BTF2P44, MGC102806, T-BTF2P44, TFIIH, p44
Protein Interaction Partner: UBC, CDK7, GTF2H5, GTF2H1, SRA1, GTF2H4, GTF2H3, UBD, COPS2, ERCC3, ERCC8, ERCC2, AR, MNAT1, CCNH,
Protein Size: 395

Molecular Weight: 44 kDa

Gene ID: 2966

NCBI Accession: NM_001515, NP_001506

UniProt: Q13888

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling