GTF2H2 antibody (N-Term)

Catalog No. ABIN6265526

This Rabbit Polyclonal antibody specifically detects GTF2H2 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GTF2H2 antibody (N-Term) (ABIN6265526)

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2H2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GTF2H2 Antibody

Binding Specificity: N-Term

Specificity: TF2H2 Antibody detects endogenous levels of total TF2H2.

Predicted Reactivity: Pig,Zebrafish,Bovine,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TF2H2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for GTF2H2

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Alternative Name: GTF2H2

Background:

Description: Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription. The N-terminus of GTF2H2 interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.

Gene: GTF2H2

Molecular Weight: 62kDa

Gene ID: 2966

UniProt: Q13888

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling