Carbonic Anhydrase IX (CA9) antibody

Antigen: Carbonic Anhydrase IX (CA9)

Synonyms: MN, CAIX, MN/CA9, CA9

Clonality: Monoclonal (1G7)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Catalog no.: ABIN411126

Additional Information

Gene ID: 768

Immunogen: Protein expressed in 293T cell transfected with human CA9 expression vector

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 1G7

Molecular Weight: 49.7 KDa

Application Details

Application Notes: WB 1:2000, IHC 1:50, Flow 1:100

Concentration: 0.5-1.0 mg/ml (Lot dependent)

Purity: Purified

Purification: Purified from mouse ascites fluids by affinity chromatography

Buffer: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Restrictions: For Research Use only

Images

Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-CA9mouse monoclonal antibody. (ABIN411126, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA9.

HEK293T cells transfected with either pCMV6-ENTRY CA9(RC204839)(Red) or empty vector control plasmid(Blue) were immunostained with anti-CA9 mouse monoclonal(ABIN411126, Dilution 1:1,000), and then analyzed by flow cytometry.