LAMP2 antibody

Catalog No. ABIN452104

This Rat Monoclonal antibody specifically detects LAMP2 in IP and IF. It exhibits reactivity toward Mouse and Rabbit.

Quick Overview for LAMP2 antibody (ABIN452104)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Mouse, Rabbit

Host: Rat

Clonality: Monoclonal

Conjugate: This LAMP2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: GL2A7

Product Details anti-LAMP2 Antibody

Specificity: The antibody detects CD107b. It will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin.

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Rabbit

Characteristics: Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2

Purification: Protein G chromatography

Immunogen: Purified preparation of mouse liver lysosomal membranes

Isotype: IgG1

Application Details

Application Notes: Immunoprecipitation (1). Immunoflourescence: 1: 500-1: 1000 (1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 0.09 % azide, in 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: CD107b / LAMP2

Background: Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cellextracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C 9$) and prostaglandin D 9@), which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2

Gene ID: 16784

UniProt: P17047

Pathways: Autophagy