CACNA1C antibody (AA 1507-1733)

Catalog No. ABIN452402

The Mouse Monoclonal anti-CACNA1C antibody is suitable to detect CACNA1C in samples from Human, Rat and Mouse. It has been validated for WB, IP, IF and IHC (fro).

Quick Overview for CACNA1C antibody (AA 1507-1733) (ABIN452402)

Target: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CACNA1C antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: S57-46

Product Details anti-CACNA1C Antibody

Binding Specificity: AA 1507-1733

Specificity: This antibody detects Cav1.2 Ca2+ Channel at ~240 kDa (varies with cell background due to glycosylation).

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Purification: Protein G chromatography

Immunogen: Fusion protein amino acids 1507-1733 (intracellular carboxyl terminus) of rabbit Cav1. 2

Isotype: IgG2b

Application Details

Application Notes: Western blot: 1-10 μg/mL (if results are off, try using thelysate without boiling). Immunoprecipitation. Immunocytochemistry: 0.1 - 1.0 μg/mL (Perox). Immunohistochemistry on frozen sections: 0.1 - 1.0 μg/mL (Perox). Immunflourescence: 1.0 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CACNA1C

Target: CACNA1C (Calcium Channel, Voltage-Dependent, L Type, alpha 1C Subunit (CACNA1C))

Alternative Name: Cav1.2 Ca2+ Channel

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on an ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifially, Cav1.2 is a cardiac L-type calcium channel, and is important for excitation and contraction of the heart (3). It may be associated with a variant of Long QT syndrome called Timothy's syndrome (4, 5) and also with Brugada syndrome. Some references also suggest it is related to bipolar disease as well (5).

Gene ID: 100101555

UniProt: P15381

Pathways: Hormone Transport, Carbohydrate Homeostasis