KCNJ2 antibody (AA 41-64, AA 189-428)

Catalog No. ABIN452411

The Mouse Monoclonal anti-KCNJ2 antibody is suitable to detect KCNJ2 in samples from Human, Mouse and Rat. It has been validated for WB and IHC (fro).

Quick Overview for KCNJ2 antibody (AA 41-64, AA 189-428) (ABIN452411)

Target: KCNJ2 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 2 (KCNJ2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNJ2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: S21-32

Product Details anti-KCNJ2 Antibody

Binding Specificity: AA 41-64, AA 189-428

Specificity: This antibody detects Kir2.1 K+ Channel at ~55 kDa. Shows no cross reactivity against Kir2.2, or Kir2.3.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Characteristics: Synonyms: KCNJ2, HIRK1, Inward rectifier K(+) channel Kir2.1, Cardiac inward rectifier potassiumchannel, IRK1, Inward rectifier potassium channel 2, Potassium channel, inwardly rectifyingsubfamily J member 2

Purification: Protein G chromatography

Immunogen: Fusion protein amino acids 41-64 and 189-428 of mouse Kir2. 1

Isotype: IgG1

Application Details

Application Notes: Western blot: 1 - 10 μg/mL (if results are off, try using the lysate without boiling). Immunohistochemistry on frozen sections: 0.1 - 1.0 μg/mL (Perox).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for KCNJ2

Target: KCNJ2 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 2 (KCNJ2))

Alternative Name: KCNJ2

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The Kir2.1 inward-rectifier potassium ion channel is encoded by the KCNJ2 gene. A defect in this gene is associated with Andersen-Tawil syndrome (3).Synonyms: Cardiac inward rectifier potassium channel, HIRK1, IRK1, Inward rectifier K(+) channel Kir2.1, Inward rectifier potassium channel 2, KCNJ2, Potassium channel, inwardly rectifying subfamily J member 2

Gene ID: 3759

UniProt: P63252