HCN4 antibody (C-Term)

Catalog No. ABIN452421

The Mouse Monoclonal anti-HCN4 antibody (Clone S114-10) (ABIN452421) specifically detects HCN4 in WB, IF and IHC (fro). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for HCN4 antibody (C-Term) (ABIN452421)

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: S114-10

Product Details anti-HCN4 Antibody

Binding Specificity: AA 1019-1108, C-Term

Specificity: This antibody detects HCN4 cyclic nucleotidegated Channel at ~130 kDa. No cross reactivity observed against other HCNs.

Cross-Reactivity (Details): Species reactivity (tested):Mouse, Rat, Human

Purification: Protein G chromatography

Immunogen: Fusion protein amino acids 1019-1108 (cytoplasmic C-terminus) of rat HCN4

Isotype: IgG1

Application Details

Application Notes: Western blot: 1-10 μg/mL (if results are off, try using thelysate without boiling). Immunocytochemistry: 0.1-1.0 μg/mL (Perox). Immunohiystochemistry on frozen sections: 0.1-1.0 μg/mL (Perox).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol and 0.09 % sodium azid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HCN4

Target: HCN4 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4 (HCN4))

Alternative Name: HCN4

Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).Synonyms: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Gene ID: 59266

UniProt: Q9JKA7