GM-CSF antibody
| Antigen |
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| Synonyms |
GMCSF, GM-CSF |
| Clonality |
Monoclonal (489) |
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Host
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Reactivity
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Application
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Western Blotting (WB) (73), Enzyme Immunoassay (EIA) (53), Flow Cytometry (FACS) (42), Immunofluorescence (IF) (40), ELISA (34), Functional Studies (Func) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (6), ELISA (Detection) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Neutralization (Neut) (4), Blocking Antibody (Inhibition) (3), ELISPOT (3), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (2), ELISA (Capture) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN452482 |
| Quantity |
0.5 mg (1.0 mg/ml) |
| Price |
410.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2
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Gene ID
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1437
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Swiss-Prot
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P04141
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Immunogen
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Purified recombinant human GM-CSF.
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Cross-Reactivity
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Human
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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489
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Description
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The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursorcells from various lineages, including granulocytes, macrophages, eosinophils anderythrocytes. Used in myeloid reconstitution following bone marrow transplant, bonemarrow transplant engraftment failure or delay, mobilization and following transplantationof autologous peripheral blood progenitor cells, and following induction chemotherapy inolder adults with acute myelogenous leukemia.
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Specificity
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This monoclonal antibody reacts with natural and recombinant human GM-CSF. Does not show any cross reaction with other human Cytokines or Growth Factors testedsuch as M-CSF, G-CSFR, IL-8, IL-16, IL1-beta, TGFbeta-1 and TNF-alpha. Species Reactivity: Tested: Human. Add. Information: Myeloma: Sp2/0-Ag14
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Application Notes
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ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml
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Purification
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Purified
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Buffer
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0.01M PBS, pH 7.2 without preservatives.Reconstitution: Restore with double distillated water is recommended and to adjust thefinal concentration to 1.00 mg/ml
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Storage
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Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Immunology, Innate Immunity, Cytokines
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Restrictions
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For Research Use only
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Alternatives for antigen "GM-CSF", type "Antibodies"
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Hosts
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Rabbit (71), Mouse (41), Rat (27), Sheep (1)
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Reactivities
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Human (83), Mouse (Murine) (49), Rat (Rattus) (28), Cow (Bovine) (19), Dog (Canine) (17), Chicken (16), Pig (Porcine) (16), Sheep (Ovine) (3), Monkey (1), Rabbit (1)
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Applications
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Western Blotting (WB) (73), Enzyme Immunoassay (EIA) (53), Flow Cytometry (FACS) (42), Immunofluorescence (IF) (40), ELISA (34), Functional Studies (Func) (8), Immunohistochemistry (Frozen Sections) (IHC (fro)) (8), Immunoprecipitation (IP) (6), ELISA (Detection) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Neutralization (Neut) (4), Blocking Antibody (Inhibition) (3), ELISPOT (3), Immunoelectron Microscopy (IEM) (2), Radioimmunoassay (RIA) (2), ELISA (Capture) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Biotin (15), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2)
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Alternatives 
