Human Immunodeficiency Virus Type 1 (HIV-1) antibody

Catalog No. ABIN452535

The Mouse Monoclonal anti-Human Immunodeficiency Virus Type 1 antibody (Clone 473) (ABIN452535) specifically detects Human Immunodeficiency Virus Type 1 in WB, EIA and ELISA (Detection). The antibody is reactive with Human Immunodeficiency Virus (HIV) samples.

Quick Overview for Human Immunodeficiency Virus Type 1 (HIV-1) antibody (ABIN452535)

Target: Human Immunodeficiency Virus Type 1 (HIV-1)

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), ELISA (Detection)

Clone: 473

Product Details

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Human immunodeficiency virus type 1, HIV-1 P24, Capsid protein p24

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified Recombinant HIV-1 P24 (Core Protein).

Isotype: IgG1

Application Details

Application Notes: ELISA: Reactivity against HIV-1 >10-5. Western Blot: Use of HIV1 P24 antibody (clone 473) at a concentration of 0.1-1.0 μg/mL willallow visualization of 100 ng/lane of recombinant HIV-1 p24.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Human Immunodeficiency Virus Type 1 (HIV-1)

Alternative Name: HIV-1

Target Type: Virus

Background: HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. P24 is expressed immediately after HIV infection and is detectable before host HIV antibody can be detected. Hence, HIV p24 is currently the target for early diagnosis of HIV acute infection.Synonyms: HIV-I, HIV1, Human immunodeficiency virus type 1