Human Immunodeficiency Virus Type 1 (HIV-1) antibody

Catalog No. ABIN452537

The Mouse Monoclonal anti-Human Immunodeficiency Virus Type 1 antibody is suitable to detect Human Immunodeficiency Virus Type 1 in samples from Human Immunodeficiency Virus (HIV). It has been validated for WB, EIA and ELISA (Capture).

Quick Overview for Human Immunodeficiency Virus Type 1 (HIV-1) antibody (ABIN452537)

Target: Human Immunodeficiency Virus Type 1 (HIV-1)

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), ELISA (Capture)

Clone: 340

Product Details

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Human immunodeficiency virus type 1, HIV-1 P24, Capsid protein p24

Purification: Affinity Chromatography on Protein G.

Immunogen: Purified Recombinant HIV-1 P24 (Core Protein).

Isotype: IgG1

Application Details

Application Notes: ELISA: This monoclonal HIV1 P24 antibody (clone 340) can be used as Capture antibody ina Sandwich ELISA for HIV P24 Detection in combination with HRP-conjugated Tracerantibody (Clone 473). Suggested Capture coating dose: 4 μg/mL for ELISA, Coating 100 μL/well, substrate: TMB. Western blot: Clear visible band at concentration of 0.1 μg/mL antibody with 0.1 μg /well ofRecombinant HIV P24 reaction.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.

Buffer: 0.01 M PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: Human Immunodeficiency Virus Type 1 (HIV-1)

Alternative Name: HIV-1

Target Type: Virus

Background: HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.Synonyms: HIV-I, HIV1, Human immunodeficiency virus type 1