EIF5A antibody (Internal Region, N-Term)

Catalog No. ABIN452675

The Goat Polyclonal anti-EIF5A antibody is suitable to detect EIF5A in samples from Human. It has been validated for WB and EIA.

Quick Overview for EIF5A antibody (Internal Region, N-Term) (ABIN452675)

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF5A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EIF5A Antibody

Binding Specificity: Internal Region, N-Term

Specificity: Recognizes eIF5A.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RPPHRASFLKRLESK, from the internal region (near N Terminus) of Human eIF5A.

Application Details

Application Notes: Peptide ELISA: eIF5A Antibody Detection limit dilution 1/128000. Western blot: 1-3 μg/mL. Approx 20 kDa band observed in MOLT4 lysates (calculated MW of20.0 kDa according to NP_001137232.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for EIF5A

Target: EIF5A (Eukaryotic Translation Initiation Factor 5A (EIF5A))

Alternative Name: EIF5A1

Background: Eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein, the only protein known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. eIF5A protein was previously reported to be involved in the first step of peptide bond formation in translation, however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.Synonyms: Eukaryotic initiation factor 5A isoform 1, Eukaryotic translation initiation factor 5A-1, Rev-binding factor, eIF-4D, eIF-5A, eIF-5A-1, eIF-5A1

Gene ID: 1984

NCBI Accession: NP_001137232

UniProt: P63241

Pathways: Regulation of Muscle Cell Differentiation