ABCD1 antibody (Internal Region)

Catalog No. ABIN452684

The Goat Polyclonal anti-ABCD1 antibody is suitable to detect ABCD1 in samples from Human. It has been validated for WB and EIA.

Quick Overview for ABCD1 antibody (Internal Region) (ABIN452684)

Target: ABCD1 (ATP-Binding Cassette, Sub-Family D (Ald), Member 1 (ABCD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCD1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-ABCD1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes ABCD1 (Internal).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EDMQRKGYSEQD, from the internal region of the protein sequence according to NP_000024.2.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/32000. Western Blot: Preliminary experiments gave an approx.50 kDa band in Human Brain(Cerebellum) lysates after 1 μg/mL antibody staining. Please note that currently we cannotfind an explanation in the literature for the band we observe given the calculated size of82.0 kDa according to NP_000024.2. The 50 kDa band was successfully blocked byincubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for ABCD1

Target: ABCD1 (ATP-Binding Cassette, Sub-Family D (Ald), Member 1 (ABCD1))

Alternative Name: ABCD1 / ALDP

Background: ABCD1 belongs to the ALD subfamily, of the superfamily of ATP-binding cassette (ABC) transporters and is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein is likely involved in the peroxisomal transport or catabolism of very long chain fatty acids. Defects in this gene have been identified as the underlying cause of adrenoleukodystrophy, an X-chromosome recessively inherited demyelinating disorder of the nervous system.Synonyms: ATP-binding cassette sub-family D member 1

Gene ID: 215

NCBI Accession: NP_000024

UniProt: P33897

Pathways: Monocarboxylic Acid Catabolic Process