TENM3 antibody (Internal Region)

Catalog No. ABIN452685

The Goat Polyclonal anti-TENM3 antibody (ABIN452685) specifically detects TENM3 in IF, IHC (p), WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for TENM3 antibody (Internal Region) (ABIN452685)

Target: TENM3 (ODZ3) (Teneurin 3 (ODZ3))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This TENM3 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TENM3 Antibody

Binding Specificity: Internal Region

Sequence: c-NNPASKIHDV KDY

Specificity: Recognizes ODZ3/Teneurin-3 (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001073946.1.

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution: 1/16000. Western Blot: Preliminary experiments in Human Brain lysates and in lysates of cell lineNIH 3T3 gave no specific signal but low background (at antibody concentration up to1 μg/mL). Immunofluorescence: lear staining (green) in HeLa cells. Immunohistochemistry: 1.5-3 μg/mL. In paraffin embedded Human Brain (Cerebellum)shows nuclear staining of the granular cell layer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TENM3

Target: TENM3 (ODZ3) (Teneurin 3 (ODZ3))

Alternative Name: Teneurin-3

Background: ODZ3 may function as a cellular signal transducer. It plays a key regulatory role in the development of aligned binocular maps, which are required for normal vision.Synonyms: KIAA1455, ODZ3, Protein Odd Oz/ten-m homolog 3, TNM3, Ten-3, Tenascin-M3

Gene ID: 55714

NCBI Accession: NP_001073946

UniProt: Q9P273