CBFB antibody (Middle Region)

Catalog No. ABIN452819

The Rabbit Polyclonal anti-CBFB antibody is suitable to detect CBFB in samples from Human. It has been validated for WB and EIA.

Quick Overview for CBFB antibody (Middle Region) (ABIN452819)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBFB antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CBFB Antibody

Binding Specificity: Middle Region

Specificity: This antibody detects CBFB at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Purified through a protein A column, followed by peptide affinity purification

Immunogen: KLH conjugated synthetic peptide selected from the Center region of human CBFB

Application Details

Application Notes: Western blot: 1: 100 - 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: CBFB

Background: CBFB is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit, it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters.Synonyms: CBF beta, CBF-beta, Core-binding factor beta, PEA2-beta, PEBP2-beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit

Molecular Weight: 21508 Da

Gene ID: 865

NCBI Accession: NP_001746

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha