DNAJB1 antibody

Catalog No. ABIN453940

This Mouse Monoclonal antibody specifically detects DNAJB1 in WB, IP, IF, EIA and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DNAJB1 antibody (ABIN453940)

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNAJB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 3B9-E6

Product Details anti-DNAJB1 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat

Characteristics: Synonyms: Heat shock 40 kDa protein 1, HSP-40, DnaJ protein homolog 1, HDJ-1, DNAJ1, HDJ1, HSPF1,DnaJ homolog subfamily B member 1

Purification: Protein G affinity chromatography

Immunogen: Recombinant protein HSP40 (HDJ1)

Isotype: IgG1

Application Details

Application Notes: Western blot. Immunoprecipitation. Immunohistochemistry on frozen sections. ELISA. Immunflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for DNAJB1

Target: DNAJB1 (DnaJ (Hsp40) Homolog, Subfamily B, Member 1 (DNAJB1))

Alternative Name: DNAJB1 / HSP40

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human Hsp40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of Hsp70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).Synonyms: DNAJ1, DnaJ homolog subfamily B member 1, DnaJ protein homolog 1, HDJ-1, HDJ1, HSP-40, HSPF1, Heat shock 40 kDa protein 1

Gene ID: 3337

UniProt: P25685