YDJ1 antibody

Catalog No. ABIN453946

This Mouse Monoclonal antibody specifically detects YDJ1 in IP, WB and EIA. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for YDJ1 antibody (ABIN453946)

Target: YDJ1 (HSP40 / YDJ1 (YDJ1))

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This YDJ1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 1G10-H8

Product Details anti-YDJ1 Antibody

No Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Species reactivity (tested):Yeast.

Purification: Protein G chromatography

Immunogen: Full length protein yeast HSP40 (YDJ1)

Isotype: IgG1

Application Details

Application Notes: Western blot: 1/2000. Immunoprecipitation. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.09 % sodium azide in 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for YDJ1

Target: YDJ1 (HSP40 / YDJ1 (YDJ1))

Alternative Name: Hsp40 / Ydj1

Background: Human Hsp40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). There are two main types of Hsp40, type 1 DNAJ proteins including HDJ2 and yeast YdjI, type II includes yeast Sis1 and human Hdj1. Whereas type I possesses a zinc finger domain which helps in the function of protein folding. (3, 4), type II does not. Members of the Hsp40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. Hsp40 stimulates the ATPase activity of Hsp70 which in turn causes conformational changes of the unfolded proteins (5, 6). The Hsp40-Hsp70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (7).

Gene ID: 850779

UniProt: P39102