SOD3 antibody

Catalog No. ABIN453955

This Mouse Monoclonal antibody specifically detects SOD3 in WB, IF, IHC (fro) and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SOD3 antibody (ABIN453955)

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SOD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 4G11G6

Product Details anti-SOD3 Antibody

Specificity: This antibody detects EC-SOD at ~35 kDa. Does not cross react with other SODs.

Cross-Reactivity (Details): Species reactivity (tested):Human, Rat, Mouse.

Characteristics: Synonyms: SOD-3, EC-SOD, Extracellular superoxide dismutase [Cu-Zn]

Purification: Protein G Chromatography

Immunogen: Human EC-SOD purified from aortas

Isotype: IgG1

Application Details

Application Notes: Western blot: 1/1000.1 μg/mL was sufficient for detection of EC-SOD in 20 μg of Human cartillage lysate bycolorimetric Immunoblot using Goat anti-Mouse IgG (HRP) as the secondary antibodyELISA (7). Immunocytochemistry: 1/100. Immunohistochemistry on Frozen Sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.

Expiry Date: 12 months

Target Details for SOD3

Target: SOD3 (Superoxide Dismutase 3, Extracellular (SOD3))

Alternative Name: Superoxide Dismutase 3 / SOD3

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2- to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2, 5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are three types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (6). SOD3 can also be distinguished by its heparin-binding capacity (1).Synonyms: EC-SOD, Extracellular superoxide dismutase [Cu-Zn], SOD-3

Gene ID: 6649

UniProt: P08294