»
Antibodies
»
anti-lambda Light Chain (Lambda-IgLC) antibody
lambda Light Chain (Lambda-IgLC) antibody
| Antigen |
|
| Clonality |
Monoclonal (4C2) |
|
Host
|
|
|
Reactivity
|
|
|
Application
|
 Alternatives Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA
Flow Cytometry (FACS) (59), Immunofluorescence (IF) (51), ELISA (48), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Immunohistochemistry (Frozen Sections) (IHC (fro)) (33), Western Blotting (WB) (27), Enzyme Immunoassay (EIA) (18), Immunohistochemistry (IHC) (14), ELISA (Capture) (2), ELISA (Detection) (2), Immunocytochemistry (ICC) (2), Immunodiffusion (ID) (2), Immunoelectrophoresis (IEP) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
|
| Catalog no. |
ABIN457412 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
286.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 3 to 5 Business Days |
|
Alternative name
|
Lambda light chains
|
|
Format
|
Purified
|
|
Isotype
|
IgG1 (Matching secondary antibodies)
|
|
Clone
|
4C2
|
|
Description
|
Immunoglobulin classes share the same basic four polypeptide chain structure of twoheavy chains (five heavy chains types) and two light chains (kappa, lambda, both having amolecular weight of 22.5kDa). Kappa and lambda consist of a variable region and aconstant region and can easily be differentiated by the antigenic properties of the constantregion. The ratio of kappa to lambda is 70:30.
|
|
Specificity
|
The antibody 4C2 reacts with lambda light chains (22.5 kDa) of human immunoglobulin. This antibody does not react with: Hamster, Sheep, Goat, Rabbit, Guinea pig.
|
|
Application Notes
|
Flow Cytometry - Positive control: RAMOS human cell line.
|
|
Concentration
|
1 mg/ml
|
|
Purity
|
> 95% (by SDS-PAGE)
|
|
Purification
|
Purified from hybridoma culture supernatant by protein-A affinity chromatography.
|
|
Buffer
|
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
|
|
Storage
|
Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "lambda Light Chain (Lambda-IgLC)", type "Antibodies"
|
Hosts
|
Mouse (74), Rabbit (49), Goat (47), Rat (7), Chicken (1), Sheep (1)
|
|
Reactivities
|
Human (146), Mouse (Murine) (36), Rat (Rattus) (7), Monkey (2)
|
|
Applications
|
Flow Cytometry (FACS) (59), Immunofluorescence (IF) (51), ELISA (48), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Immunohistochemistry (Frozen Sections) (IHC (fro)) (33), Western Blotting (WB) (27), Enzyme Immunoassay (EIA) (18), Immunohistochemistry (IHC) (14), ELISA (Capture) (2), ELISA (Detection) (2), Immunocytochemistry (ICC) (2), Immunodiffusion (ID) (2), Immunoelectrophoresis (IEP) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
|
|
Conjugates
|
FITC (21), HRP (14), Biotin (11), PE (9), Alkaline Phosphatase (AP) (7), RPE (6), Texas Red (TR) (5), TRITC (4), APC (3), Rhodamine (3), Al-Cy (1), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alexa Fluor 700 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
|
|
Epitopes
|
N-Term (1)
|
