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beta-Catenin (CTNNB1) antibody

Antigen

beta-Catenin (CTNNB1)
Synonyms
CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1) ... show more
Clonality Monoclonal (EM-22)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Mouse (Murine), Hamster
Application
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)
Catalog no. ABIN457419
Quantity 0.1 mg  (1 mg/ml)
Price 325.00 $   Plus shipping costs $35.00
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Additional Information
Application Details Alternatives Secondary Antibodies back to top

Alternative name beta-Catenin
Immunogen Recombinant human beta-catenin
Format Purified
Isotype IgG1  (Matching secondary antibodies)
Clone EM-22
Description Beta-catenin is a multifunctional protein involved both in cell adhesion and in activation oftranscription. Calcium-dependent intercellular adhesion transmembrane glycoproteinE-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta andgamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton.Functional cadherin-catenin system is important for invasiveness of tumour cells.Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. Whenactivity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway),hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocatesto the nucleus and activates transcription of genes including those that are involved in cellcycle control. As a result, cell division and neoplastic transformation are promoted.
Specificity The antibody EM-22 reacts with C-terminal part of beta-catenin, an 88 kDa multifunctionalprotein involved both in cell adhesion and in activation of transcription.
Synonyms CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Application Details
Alternatives Secondary Antibodies back to top

Application Notes Western Blotting - Positive control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1human colon adenocarcinoma cell lineKW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line. Immunocytochemistry: Positive control: HT29 human colon adenocarcinoma cell line
Concentration 1 mg/ml
Purity > 95% (by SDS-PAGE)
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Storage Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
Restrictions For Research Use only

Alternatives
Application Details Secondary Antibodies back to top

Alternatives for antigen "beta-Catenin (CTNNB1)", type "Antibodies"
Hosts Rabbit (149), Mouse (41)
Reactivities Human (173), Mouse (Murine) (113), Rat (Rattus) (105), Chicken (18), Dog (Canine) (6), Xenopus laevis (2), Hamster (1), Monkey (1)
Applications Western Blotting (WB) (169), Immunofluorescence (IF) (56), Immunoprecipitation (IP) (46), Enzyme Immunoassay (EIA) (43), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (43), Immunohistochemistry (IHC) (36), ELISA (27), Flow Cytometry (FACS) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (12), Dot Blot (Dot) (7), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Biotin (5), FITC (2), HRP (2), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
Epitopes pSer37 (9), pSer33 (8), Ab-33 (5), Ab-37 (5), Ab-41/45 (5), C-Term (5), pThr41/Ser45 (5), pThr41/pSer45 (4), Ab-654 (3), pTyr654 (3), pTyr86 (3), exon 3 (2), pSer33/37 (2), Ab-489 (1), Core (1), dep27-37 (1), dep35-50 (1), pSer45 (1), pTyr489 (1)