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Antibodies
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anti-Vascular Cell Adhesion Molecule 1 (VCAM1) antibody
Vascular Cell Adhesion Molecule 1 (VCAM1) antibody
| Antigen |
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| Synonyms |
CD106, MGC99561, INCAM-100, DKFZp779G2333, Vcam-1, VCAM1B, MGC108734, VCAM1, fi76d06, wu:fi76d06, zgc:158875, VCAM-1, DKFZp469N1820 |
| Clonality |
Monoclonal (STA) |
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Host
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Reactivity
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Application
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 Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA
Flow Cytometry (FACS) (158), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunoprecipitation (IP) (47), Western Blotting (WB) (38), Immunofluorescence (IF) (35), ELISA (29), Immunohistochemistry (IHC) (24), Functional Studies (Func) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Enzyme Immunoassay (EIA) (11), Blocking Antibody (Inhibition) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN457435 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
260.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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CD106
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Immunogen
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Human DS6 T cell line
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Format
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Purified
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Isotype
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IgG1 (Matching secondary antibodies)
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Clone
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STA
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Description
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CD106 / VCAM-1 (vascular cell adhesion molecule-1) is an Ig-like cell surface adhesionmolecule binding VLA-4 integrin. VCAM-1 is a potent T cell costimulatory molecule takingpart in their positive selection and survival, as well as in adhesion, transendothelial migrationand activation of peripheral T cells. VCAM-1 is also involved in endothelial cell-cell contacts.Whereas VCAM-1 normally mediates leukocyte extravasion to sites of tissue inflammation,tumour cells can use overexpressed VCAM-1 to escape T cell immunity. Soluble form ofVCAM-1 (sVCAM-1) is an inflammatory marker and can be used also in prognosis ofsubsequent cariovascular events following acute coronary syndromes.
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Specificity
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The mouse monoclonal antibody STA recognizes CD106 antigen (VCAM-1), a 100-110 kDatype I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyteadhesion, and a costimulation molecule.HLDA V, WS Code A013
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Application Notes
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Flow Cytometry: Recommended dilution: 6 µg/ml. Positive control: TNF-alpha activated HUVEC cellsImmunohistochemistry (frozen sections). Application note: acetone fixationELISA. Application note: capture mAb for soluble CD106
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Concentration
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1 mg/ml
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Purity
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> 95% (by SDS-PAGE)
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Purification
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Purified from ascites by protein-A affinity chromatography.
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Alternatives for antigen "Vascular Cell Adhesion Molecule 1 (VCAM1)", type "Antibodies"
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Hosts
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Mouse (114), Rat (62), Rabbit (26), Sheep (2)
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Reactivities
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Human (124), Mouse (Murine) (81), Rat (Rattus) (29), Monkey (5), Pig (Porcine) (5), Dog (Canine) (4), Chicken (2), Cow (Bovine) (2), Horse (Equine) (2), Rabbit (2)
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Applications
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Flow Cytometry (FACS) (158), Immunohistochemistry (Frozen Sections) (IHC (fro)) (56), Immunoprecipitation (IP) (47), Western Blotting (WB) (38), Immunofluorescence (IF) (35), ELISA (29), Immunohistochemistry (IHC) (24), Functional Studies (Func) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), Enzyme Immunoassay (EIA) (11), Blocking Antibody (Inhibition) (4), Blocking Antibody (Inhibition) (3), Immunocytochemistry (ICC) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (30), FITC (27), PE (17), RPE (4), APC (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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