Immunoglobulin E (IgE) antibody (Biotin)

Antigen: Immunoglobulin E (IgE)

Clonality: Monoclonal (BSW17-NBS01)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)

Catalog no.: ABIN457503

Additional Information

Gene ID: IGHE, chromosome 14q32.33

Immunogen: purified human myeloma IgE

Format: Affinity-purified

Isotype: IgG1 kappa

Clone: BSW17-NBS01

Description: The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.

Specificity: Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes

Sensitivity: Reactivity > 95%, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)

Application Details

Application Notes: Sterile filtered solution, does not contain Na-azide. Recommended for functional cellular assays (mast cell / basophil activation).

Concentration: 1mg/ml, Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Purity: > 85%, tested by analytical Gel Permeation Chromatography

Purification: Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – rat monoclonal anti- mouse Ck

Buffer: PBS, pH=7.2, sterile filtered, contains no NaN3

Storage: Store undiluted at 4 °C. For long term storage, aliquot and store at -20°C or -80°C

Research Area: Allergy

Restrictions: For Research Use only

Images

product purity: gel permeation chromatography (Superose 12/HR)

ELISA binding to human IgE(SUS-11), Detection: Streptavidin-HRP