alpha and beta Lipoproteins antibody

Catalog No. ABIN457940

The Goat Polyclonal anti-alpha and beta Lipoproteins antibody is suitable to detect alpha and beta Lipoproteins in samples from Human. It has been validated for IEP.

Quick Overview for alpha and beta Lipoproteins antibody (ABIN457940)

Target: alpha and beta Lipoproteins

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP)

Product Details

Specificity: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. of this antiserum has not been tested in detail.

Characteristics: Precipitating polyclonal goat antiserum to human alpha and beta lipoproteins

Purification: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Lipoproteins form a heterogeneous system of protein-lipid-carbohydrate complexes which provides highly efficient transport of phospholipids, glycolipids, fatty acids, mono-, di- and triglycerides, cholesterol and cholesterolesters. Concentrations in normal human serum is 230-490 mg/100 ml. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: In precipitating techniques as immunoelectrophoresis and single or double radial immuno-diffusion to identify the presence of alpha-1lipoprotein in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Antibody titre: Precipitin titre 1:64 when tested against in agar-block immunodiffusion titration against an appropriate

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Preservative: Without preservative

Storage: 4 °C

Target Details

Target: alpha and beta Lipoproteins

Background: The reactivity of the antiserum is restricted to lipoproteins. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma three precipitin lines are obtained. No reaction is obtained with any other plasma protein component or serum