Sheep anti-Mouse IgG2a (Allotype 1b), (Allotype Specific) Antibody

Catalog No. ABIN458534

Quick Overview for Sheep anti-Mouse IgG2a (Allotype 1b), (Allotype Specific) Antibody (ABIN458534)

Target: IgG2a

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)

Product Details

Binding Specificity: Allotype 1b, Allotype Specific

Reactivity: Mouse

Host: Sheep

Specificity: Purified IgG fraction of polyclonal Sheep antiSerum to Mouse IgG2a(1b), allotype specific.

Cross-Reactivity (Details): The antiSerum does not cross-react with any other component of the Mouse Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, expected chance of cross-reactivity will be small.

Characteristics: The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1b, including C57Bl and SJL/J, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1b). No precipitation reaction is obtained with purified IgG2a(1a) (Balb/C, CBA, AKR and NZB), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. As unlabelled primary or secondary reagent for indirect detection of IgG2a(1b) at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.

Purification: Purified

Immunogen: Highly purified normal homogenous IgG2a(1b) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune IgG fraction, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgG2a

Target Type: Antibody