Goat anti-Rat IgA, IgG, IgM (Fc Region) Antibody (FITC) - Preadsorbed

Catalog No. ABIN458687

Quick Overview for Goat anti-Rat IgA, IgG, IgM (Fc Region) Antibody (FITC) - Preadsorbed (ABIN458687)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: FITC

Application: Immunofluorescence (IF)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Rat

Host: Goat

Specificity: The reactivity of the antiserum is directed to the Fc subunits of the major isotypes of the rat immunoglobulin system. No reaction is obtained with purified IgG/Fab or any non-Ig protein of rat serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal rat plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained

Characteristics: Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to rat IgG, IgA and IgM, Fc specific
Fluorochrome/IgG protein molar ratio (F/P) is approximately 2.0.

Purification: Preadsorption: Immunoaffinity adsorbed using insolubilized Ig-depleted rat serum fractions and IgG/Fab as required to eliminate antibodies reacting with the common Fab portion of immunoglobulins or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.

Immunogen: Purified polyclonal IgG and homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates, to demonstrate immunoglobulins or specific antibodies in cells and tissues, to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. The absence of activity to the common Ig/Fab subunit prevents the reaction of this conjugate with immunoglobulins bounds to Fc receptors on non-lymphoid cells. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Concentration: 10 mg/mL

Buffer: FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Preservative: Without preservative

Handling Advice: P rior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and prefer ably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM

Target Type: Antibody