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Details for Product No. ABIN459320

Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody

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Antigen
Synonyms
UVO, CDHE, ECAD, LCAM, Arc-1, CD324, arc-1, cd324, cdhe, ecad, lcam, uvo, AA960649, E-cad, Ecad, L-CAM, Um, E-cadherin, E-Cad, XB-cadherin, XBcad, XTCAD-1, b-cadherin, cadherin-1, cdh1-a, cdhc-A, cdhp ... show more
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
Pubmed 4 references available
Catalog no. ABIN459320
Quantity 100 µg
Price
396.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 5 to 7 Business Days
Immunogen 5H9 is a mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 mouse myeloma cells with spleen cells from a BABL/c mouse immunized with affinity purified 80 kD extracellular fragments of E-cadherin derived from tryptic digestion of A-431 human vulva carcinoma cells.
Clone 5H9
Isotype IgG1
Specificity 5H9 recognizes both the 120 kD E-cadherin and its 80 kD trypsin-resistant extracellular part.
Purity Affinity purified
Alternative Name E-Cadherin/Cadherin-1
Background Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. They function in the maintenance of tissue integrity and morphogenesis. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin or cadherin-3) and retinal cadherin (R-cadherin or cadherin-4), whereas kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. One of the best characterized cadherins is E-cadherin, a 120 kD transmembrane glycoprotein consisting of an 80 kD extracellular and a 40 kD transmembrane and cytoplasmic part. The extracellular domains of E-cadherin are responsible for calcium binding which allows for homophilic interaction with other E-cadherin molecules on the same cell and neighbouring cells. In addition, E-cadherin can interact heterophilically with integrin E7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through the associated cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.
Application Notes 5H9 is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen and paraffin embedded tissues. For frozen tissues use a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For paraffin embedded tissues use a pretreatment step of 15 minutes incubation in TRIS-EDTA buffer pH 9 in a microwave. Recommended dilution Optimal antibody dilution should be determined by titration, recommended range is 1:50 – 1:100 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting applications.
Restrictions For Research Use only
Format Liquid
Concentration 1mg/ml
Buffer Each vial contains 100 ml of 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
Preservative Sodium azide
Storage 4 °C
Frixen, Behrens, Sachs et al.: "E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells." in: The Journal of cell biology, Vol. 113, Issue 1, pp. 173-85, 1991 (PubMed).

Moll, Mitze, Frixen et al.: "Differential loss of E-cadherin expression in infiltrating ductal and lobular breast carcinomas." in: The American journal of pathology, Vol. 143, Issue 6, pp. 1731-42, 1994 (PubMed).

Gabbert, Mueller, Schneiders et al.: "Prognostic value of E-cadherin expression in 413 gastric carcinomas." in: International journal of cancer. Journal international du cancer, Vol. 69, Issue 3, pp. 184-9, 1996 (PubMed).

Schwechheimer, Zhou, Birchmeier: "E-Cadherin in human brain tumours: loss of immunoreactivity in malignant meningiomas." in: Virchows Archiv : an international journal of pathology, Vol. 432, Issue 2, pp. 163-7, 1998 (PubMed).

Alternatives for antigen "Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)", type "Antibodies"
Hosts (131), (122), (9), (5), (4)
Reactivities (238), (83), (55), (34), (34), (24), (20), (19), (15), (14), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Applications (176), (76), (61), (60), (43), (34), (31), (30), (30), (26), (12), (8), (6), (3), (2), (1), (1), (1)
Conjugates (10), (7), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Epitopes (11), (9), (8), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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