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E-cadherin antibody (AA 155-707)

Name: E-cadherin antibody (AA 155-707)
Brand: antibodies-online
SKU: ABIN7146194
Price: 385 USD
Availability: InStock

CDH1 Reactivity: Human WB, FACS, ELISA, IF Host: Mouse Monoclonal 9H2D3 unconjugated

Western Blot Positive WB detected in: Caco205 whole cell lysate All lanes: CDH1 antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 98, 91 kDa Observed band size: 110, 120 kDa (E-cadherin antibody (AA 155-707))

Overlay histogram showing MCF-7 cells stained with ABIN7146194 (red line) at 1:100. (E-cadherin antibody (AA 155-707))

FACS

Immunofluorescence staining of Hela cells with ABIN7146194 at 1:100, counter-stained with DAPI. (E-cadherin antibody (AA 155-707))

Immunofluorescence staining of MCF-7 cells with ABIN7146194 at 1:100, counter-stained with DAPI. (E-cadherin antibody (AA 155-707))

Immunofluorescence staining of SW620 cells with ABIN7146194 at 1:100, counter-stained with DAPI. (E-cadherin antibody (AA 155-707))

5 images

Catalog No. ABIN7146194

Datasheet as PDF

Target See all E-cadherin (CDH1) Antibodies

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
Binding Specificity
17

17

16

11

10

10

8

8

7

7

5

4

4

4

4

4

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 155-707
Reactivity
225

96

83

18

17

12

9

9

9

6

4

4

4

4

1

1

1

1

1

1
Human
Host
193

71

7

3
Mouse
Compatible Secondaries
Clonality
179

94

1
Monoclonal
Conjugate
134

34

28

8

7

6

6

6

6

6

6

6

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This E-cadherin antibody is un-conjugated
Application
192

88

81

63

53

49

39

35

29

27

17

16

5

5

4

4

3

3

2

1

1

1
Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Cross-Reactivity

Human, Mouse

Purification

Protein G purified

Immunogen

Recombinant Human Cadherin-1 protein (155-707AA)

Clone

9H2D3

Isotype

IgG1, IgG1 kappa

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Application Notes

Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name

CDH1 (CDH1 Products)

Synonyms

Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

Background

Background: Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Aliases: Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3], CDH1, CDHE UVO

UniProt

P12830

Pathways

WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation