E-cadherin antibody

Catalog No. ABIN1440031

This Goat Polyclonal antibody specifically detects E-cadherin in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Pig, Monkey, Chicken, Dog, Rabbit, Cow, Horse, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster and Sheep. It has been mentioned in 3+ publications

Quick Overview for E-cadherin antibody (ABIN1440031)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse, Rat, Pig, Monkey, Chicken, Dog, Rabbit, Cow, Horse, Guinea Pig, Avian, Cat, Donkey, Goat, Hamster, Sheep

Host: Goat

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-E-cadherin Antibody

Purpose: Anti-CDH1

Sequence: YYYDEEGGGE EDQDFDLSQL HRGLDARPEV TRNDVAPTLM SVPRYLPRPA NPDEIGNFID ENLKAADTDP TAPPYDSLLV FDYEGSGSEA ASLSSL

Specificity: Detects endogenous levels of total E-cadherin protein by Western blot in the whole cell lysates (COS-7 and MDCK).

Characteristics: E-Cadherin

Purification: Epitope affinity purified

Immunogen: Purified recombinant peptide derived from within residues 750 to 850 of human CDH1 produced in E. coli.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:2,000, IHC-P:1:250-1:1,000, IHC-F:1:250-1:1,000

Restrictions: For Research Use only

Handling

Concentration: 2 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C,-20 °C

Storage Comment: For continuous use, store at 2-8 C for one-two days. For extended storage, store in -20 C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-E-cadherin antibody (ABIN1440031)

Berardinelli, Russo, Canciello, Di Giacinto, Mauro, Nardinocchi, Bove, Solari, Del Basso De Caro, Cavallo, Barboni: "KLHL14 and E-Cadherin Nuclear Co-Expression as Predicting Factor of Nonfunctioning PitNET Invasiveness: Preliminary Study." in: Journal of clinical medicine, Vol. 13, Issue 15, (2024) (PubMed).

Rapino, Castellucci, Lizzi, Sabatucci, Angelucci, Tortolani, Rossi, DAndrea, Maccarrone: "Modulation of Endocannabinoid-Binding Receptors in Human Neuroblastoma Cells by Tunicamycin." in: Molecules (Basel, Switzerland), Vol. 24, Issue 7, (2019) (PubMed).

Canciello, Russo, Berardinelli, Bernabò, Muttini, Mattioli, Barboni: "Progesterone prevents epithelial-mesenchymal transition of ovine amniotic epithelial cells and enhances their immunomodulatory properties." in: Scientific reports, Vol. 7, Issue 1, pp. 3761, (2018) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1

Background:

Goat polyclonal to CDH1. CDH1 is a classical member of the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised consists of 5 cadherin repeats in the extracellular domain, one trans membrane domain, and a and a highly conserved intracellular domain that binds b-catenin and p120-catenin. CDH1 is involved in mechanisms regulating proliferation, mobility and cell-cell adhesions of epithelial cells. Loss of E-cadherin function or expression has been implicated in cancer metastasis and progression.

ARC-1, CAM 120/80, CD324, CDH1, cdhc-A, CDHE, ECAD, E-Cad E-cadherin, Epithelial cadherin, l-cam, LCAM, P09803, P12830 / Q9R0T4, UVO, Uvomorulin, XBcad, XB-cadherin, XTCAD-1 antibody.

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation