S100A10 antibody

Catalog No. ABIN462133

This Mouse Monoclonal antibody specifically detects S100A10 in WB, IHC, ELISA, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for S100A10 antibody (ABIN462133)

Target: S100A10 (S100 Calcium Binding Protein A10 (S100A10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4E7E10

Product Details anti-S100A10 Antibody

Specificity: Ni-NTA purified truncated recombinant P11 expressed in E. Coli strain BL21 (DE3)

Purification: Protein A purified

Immunogen: Purified recombinant fragment of human P11 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200), WB (1:100 - 1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:200.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Ascitic fluid containing 0.03 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: -20°C.

Target Details for S100A10

Target: S100A10 (S100 Calcium Binding Protein A10 (S100A10))

Alternative Name: S100A10

Background: Name/Gene ID: S100A10

Synonyms: S100A10, 42C, ANX2LG, Calpactin-1 light chain, Calpactin I light chain, CLP11, p11, p10, p10 protein, Protein S100-A10, ANX2L, Ca[1], CAL1L, Cellular ligand of annexin II, gp11

Gene ID: 6281

UniProt: P60903

Pathways: S100 Proteins