TCF7L1 antibody (AA 35-84)

Catalog No. ABIN6741527

This Rabbit Polyclonal antibody specifically detects TCF7L1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Rabbit and Guinea Pig.

Quick Overview for TCF7L1 antibody (AA 35-84) (ABIN6741527)

Target: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCF7L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TCF7L1 Antibody

Binding Specificity: AA 35-84

Specificity: Human TCF7L1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Guinea pig (100%) Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa35-84 of human TCF7L1 (Q9HCS4, NP_112573). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Galago, Marmoset, Mouse, Rat, Bovine, Guinea pig (100%), Monkey, Elephant, Opossum (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TCF7L1

Target: TCF7L1 (Transcription Factor 7-Like 1 (T-Cell Specific, HMG-Box) (TCF7L1))

Alternative Name: TCF7L1

Background: Name/Gene ID: TCF7L1

Synonyms: TCF7L1, TCF-3, Transcription factor 7-like 1, HMG box transcription factor 3

Gene ID: 83439

NCBI Accession: NP_112573

UniProt: Q9HCS4

Pathways: WNT Signaling, Stem Cell Maintenance