TGFB1I1 antibody (N-Term)

Catalog No. ABIN6741575

This Rabbit Polyclonal antibody specifically detects TGFB1I1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Rat, Mouse, Dog and Rabbit.

Quick Overview for TGFB1I1 antibody (N-Term) (ABIN6741575)

Target: TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))

Reactivity: Human, Rat, Mouse, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFB1I1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TGFB1I1 Antibody

Binding Specificity: N-Term

Specificity: Human TGFB1I1 / HIC-5

Predicted Reactivity: Percent identity by BLAST analysis: Mouse, Rat, Dog, Rabbit (100%) Horse (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human TGFB1I1 (B2R8D5, NP_057011). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Mouse, Rat, Panda, Dog, Rabbit (100%), Marmoset, Horse (92%), Bat (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Tested in Western blot using transfected cells. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TGFB1I1

Target: TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))

Alternative Name: ARA55 / HIC-5

Background: Name/Gene ID: TGFB1I1
Family: TGF beta

Synonyms: TGFB1I1, ARA55, HIC5, HIC-5, TSC-5

Gene ID: 7041

NCBI Accession: NP_057011

UniProt: O43294

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, VEGF Signaling