CYCS / Cytochrome c antibody

Antigen: CYCS / Cytochrome c

Clonality: Monoclonal (7H8.2C12)

Host: Mouse

Reactivity: Horse (Equine)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN462359

Additional Information

Swiss-Prot: P99999

Immunogen: Protein, Horse cytochrome c.

Isotype: IgG2b  (Matching secondary antibodies)

Clone: 7H8.2C12

Description: Gene: CYCS / Cytochrome c, Standard Gene Symbol: CYCS, Synonyms: CYCS, CYC, Cytochrome c, HCS, THC4

Specificity: Detects an ~14kD protein, corresponding to the apparent molecular mass of Cytochrome c on Western blots with samples from human, mouse, rat, bovine, guinea pig, hamster, monkey, rabbit and sheep origins. This antibody detects denatured Cytochrome c in applications such as Western blot, it does not recognize the native protein. The epitope of this antibody was mapped to a region within amino acids 93-104 of horse Cytochrome. Predicted Reactivities: Cow (Bovine),Guinea Pig,Hamster,Human,Monkey,Mouse (Murine),Pig (Porcine),Rabbit,Rat (Rattus),Sheep (Ovine).

Application Details

Application Notes: IHC-P (5 µg/ml), WB (1 µg/ml)

Concentration: 1 mg/ml

Purification: Protein G Column

Buffer: PBS, pH 7.2, 50% glycerol, 0.09% azide

Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Restrictions: For Research Use only

Images

Human Uterus (formalin-fixed, paraffin-embedded) stained with CYCS antibody ABIN462359 followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.

Human Spleen (formalin-fixed, paraffin-embedded) stained with CYCS antibody ABIN462359 followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.

Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with CYCS antibody ABIN462359 followed by biotinylated anti-mouse IgG secondary antibody ABIN481714, alkaline phosphatase-streptavidin and chromogen.