USP9X antibody (AA 2479-2492)

Catalog No. ABIN462529

This Goat Polyclonal antibody specifically detects USP9X in IHC and IHC (p). It exhibits reactivity toward Human and Monkey.

Quick Overview for USP9X antibody (AA 2479-2492) (ABIN462529)

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This USP9X antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-USP9X Antibody

Binding Specificity: AA 2479-2492

Specificity: Human USP9X. This antibody is expected to recognize isoform 3 (NP_001034679.2)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant (100%) Rabbit (93%) Mouse, Horse (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-KKATSVQQIEMEES from the C-terminus (near) of human USP9X (NP_001034679.2). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant (100%), Rabbit (93%), Mouse, Horse (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for USP9X

Target: USP9X (Ubiquitin Specific Peptidase 9, X-Linked (USP9X))

Alternative Name: USP9X / FAM

Background: Name/Gene ID: USP9X
Subfamily: Cysteine C19
Family: Protease

Synonyms: USP9X, DFFRX, FAM, Fat facets in mammals, HFAM, Ubiquitin thioesterase FAF-X, USP9, Deubiquitinating enzyme FAF-X, FAF, Ubiquitin thiolesterase FAF-X

Gene ID: 8239

NCBI Accession: NP_001034679

UniProt: Q93008