PKD1 antibody (AA 4289-4300)

Catalog No. ABIN462530

The Goat Polyclonal anti-PKD1 antibody is suitable to detect PKD1 in samples from Human. It has been validated for IHC and IHC (p).

Quick Overview for PKD1 antibody (AA 4289-4300) (ABIN462530)

Target: PKD1 (Polycystic Kidney Disease 1 (Autosomal Dominant) (PKD1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PKD1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKD1 Antibody

Binding Specificity: AA 4289-4300

Specificity: Human PKD1. This antibody is expected to recognize both reported isoforms (NP_001009944.1 and NP_000287.2).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Marmoset, Panda, Cat, Dog, Horse (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RTPLRAKNKVHP from the C-terminus of human PKD1 (NP_001009944.1, NP_000287.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Marmoset, Panda, Cat, Dog, Horse (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PKD1

Target: PKD1 (Polycystic Kidney Disease 1 (Autosomal Dominant) (PKD1))

Alternative Name: PKD1 / Polycystin

Background: Name/Gene ID: PKD1

Synonyms: PKD1, PBP, Polycystin, TRPP1, Pc-1, Polycystin 1, Polycystin-1

Gene ID: 5310

NCBI Accession: NP_001009944, NP_000287

UniProt: P98161

Pathways: Myometrial Relaxation and Contraction, Maintenance of Protein Location